Composite

Part:BBa_K3037005:Design

Designed by: Arnau Pérez Roig   Group: iGEM19_TU_Dresden   (2019-10-14)


MBP+eGFP+dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3061
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
    Illegal BamHI site found at 1930
    Illegal BamHI site found at 5340
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


Design Notes

dCas9 mutated for remove EcoRI


Source

IDT

References